Mouse intratracheal instillation
Nettet2. des. 2024 · Intratracheal instillation infection. Mice (n = 20) were anaesthetized with isoflurane and then suspended by their upper incisors from a nylon string on an angled plexiglass platform. Once the tracheal opening was visualized using the laryngoscope, the delivery tube was inserted gently into the trachea of the mouse. NettetRodent lungs are routinely examined after intratracheal instillation (IT) of fixative. This study compares the histopathologic appearance of the lung after IT fixation with air inflation (AI) followed by immersion fixation. Lungs from mice chronically exposed to cigarette smoke were fixed either by IT or by AI.
Mouse intratracheal instillation
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Nettet9. jun. 2014 · In the rat quartz instillation model, male 10-week old F344 rats were exposed by intratracheal instillation (IT) to quartz at a dose of 2 mg/rat suspended in saline (0.2 ml) on day 0, and sacrificed on day 28. Lung tumorigenesis in F344 male rats was initiated by DHPN in drinking water for 2 weeks, and the animals were then … NettetFigure 1 Pulmonary ZnONPs exposure caused the pathological changes in mouse liver. (A) After a single treatment of 3, 6, 12 μg/animal ZnONPs via tracheal instillation, H&E staining on liver tissues of mice showed a dose-related manner on pathological changes.(B) The mice were treated with 12 μg/animal ZnONPs via tracheal …
NettetIntratracheal instillation of fluorescent compound, Cy5.5 conjugated to dextran, shows successful instillation of fluorescent compound in B6 mouse lung (Figure 1). The fluorescent dye is primarily targeted to the lung and is evenly distributed. Fluorescent signal in the gastrointestinal NettetIntratracheal instillation is the introduction of a substance directly into the trachea. It is widely used to test the respiratory toxicity of a substance as an alternative to inhalation in animal testing. [1] Intratracheal instillation was reported as early as 1923 in studies of the carcinogenicity of coal tar.
Nettet15. mai 2024 · The intratracheal injection technique requires a skin incision, which will begin to heal immediately following closure. If re-administration is required within a relatively short timeframe, the same incision site could be … NettetControl mice received an intratracheal injection of 50 μL of saline. After the intratracheal injection, the mice were placed in a vertical position and rotated for 1 min to distribute the instillation evenly within the lungs. Forty-eight hours after the intratracheal injection, the animals were sacrificed. Blood and tissue samples were harvested.
Nettet8. apr. 2024 · Our present findings showed that tree shrew models of ARDS triggered by a one-hit intratracheal instillation of LPS reproduced many of the characteristics of the acute phase of ARDS in human patients, such as prolonged endpoints, obvious symptoms of dyspnea, intensive inflammatory infiltration, and the formation of a hyaline membrane …
Nettetflow of liquid during the instillation of APC or vehicle into the trachea lumen. The skin wound was then closed and the animals were allowed to recover. The optimal day for the treatment of lung injury with APC was evaluated by treating mice with a single intratracheal instillation of APC (1 mg/kg of mouse body weight; Sigma, St. Louis, … rayshon meaningNettetEngineered nanomaterials (ENMs) are products of the emerging nanotechnology industry and many different types of ENMs have been shown to cause chronic inflammation in the lungs of rodents after inhalation exposure, suggesting a risk to human health. Due to the increasing demand and use of ENMs in a variety of products, a careful evaluation of the … rayshon hunter sandusky ohiorayshon loveNettetThis short video shows a simple quick and easy method to intubate mice. The procedure can be used for either instillation of drugs or chemicals, or for measurement of lung function. Show more ... rayshon holmesNettet2. aug. 2010 · GFP expression in bronchial epithelium of mice infected with the LV vector. PBS (a) or the LV-GFP vector (b) was injected in the lung of C57BL/6 mice through an intratracheal instillation. GFP expression was evaluated 1 month later by immunohistochemistry. simply divine by marilynNettet11. feb. 2016 · Biodistribution and clearance of 14 C-graphene in male ICR mice after a intratracheally instillation or b oral gavage (5 μg 14 C-graphene exposed) at different time points. c Raman spectra of the FLG obtained from stomach, small intestine, large intestine and lung after exposure for 1 d. Mouse feces were collected by metabolism cages. … simply divine botanicals amazing faceNettet14. apr. 2024 · A total of 50 µL of PMWCNT and TMWCNT suspensions were administered via intratracheal instillation to the mice in doses of 10 or 100 µg/mouse. After being anesthetized intra-peritoneally with ketamine/xylazine (150/15 mg/kg mixture) solution, a 24-gauge catheter was intubated into the trachea. simply divine botanicals